Article de revue
Rabies vaccine preserved by vaporization is thermostable and immunogenic
A rabies vaccine that is thermostable over a range of ambient environmental temperatures would be highly advantageous, especially for tropical regions with challenging cold-chain storage where canine rabies remains enzootic resulting in preventable human mortality. Live attenuated rabies virus (RABV) strain ERAG333 (R333E) was preserved by vaporization (PBV) in a dry, stable foam. RABV stabilized using this process remains viable for at least 23 months at 22 °C, 15 months at 37 °C, and 3 h at 80 °C. An antigen capture assay revealed RABV PBV inactivated by irradiation contained similar levels of antigen as a commercial vaccine. Viability and antigen capture testing confirmed that the PBV process stabilized RABV with no significant loss in titer or antigen content. Live attenuated and inactivated RABV PBV both effectively induced RABV neutralizing antibodies and protected mice from peripheral RABV challenge. These results demonstrate that PBV is an efficient method for RABV stabilization.
Auteurs
Langues
- Anglais
Année de publication
2015
Journal
Vaccine
Volume
19
Type
Article de revue
Catégories
- Vaccins et dispositifs d'administration
Maladies
- Rage
Mots-clés
- Distribution system
- New vaccine introduction