Guidance

Supplement 2 to the WHO Polio Laboratory Manual Adaptation of newly received cells to local conditions

Critical steps in assuring quality cell cultures and reproducibility of cell line stocks occur at the time of receipt of new cultures in the laboratory and establishment of a frozen cell bank. Experiences in the Global Polio Laboratory Network (GPLN) suggest that several factors can affect the adaptation of newly received cells to the local conditions of the laboratory. These include differences in cell culture conditions that exist between the source and the recipient laboratory (e.g. media composition, culture vessels and type of incubator), transportation conditions (e.g. temperature fluctuation, mishandling), presence of contaminants, regimen for medium replenishment, and techniques for cell handling. For successful adaptation of the cells, it is important that the recipient laboratory has information, as specified in Section S2.1 below, about the cells it receives so that there can be a systematic approach to troubleshooting should problems occur. Information should be kept on file for future reference with the date received. The details of observations and procedures carried out during the cell adaptation process should be recorded, as they are generated, and archived for future reference.

Languages

  • English

Publisher

WHO

Type

Guidance

Categories

  • Programme management

Diseases

  • Polio

Organisations

  • WHO

Topic references

EPI-CORE-POLIO

TitleAuthorYearTypeLanguage
Classification and reporting of vaccine-derived polioviruses ((VDPV)GPEI - Global Polio Eradication Initiative 2016GuidanceEnglish
Containment Certification Scheme to support the WHO Global Action Plan for Poliovirus Containment (GAPIII-CCS)World Health Organization (WHO)1960RepositorySpanish
Fractional dose IPV decision-making summary guideWorld Health Organization (WHO)RepositoryFrench
Global Polio Eradication Initiative (GPEI) Tools, Protocols and GuidelinesWorld Health Organization (WHO)RepositoryEnglish
Guide to introducing IPV vaccine into national immunization programmesWorld Health Organization (WHO)2018RepositoryFrench
Polio Eradication and Endgame Strategic Plan 2013-2018World Health Organization (WHO)2013GuidanceFrench
Polio laboratory manual, 4th editionWorld Health Organization (WHO)2004GuidanceEnglish
Polio post-certification strategyGPEI - Global Polio Eradication Initiative 2018GuidanceEnglish
Polio vaccines: WHO position paper – March, 2016World Health Organization (WHO)GuidanceArabic, Chinese, English, French, Russian, Spanish
SOP: Responding to a Poliovirus Outbreak or Event (Parts 1 and 2)GPEI - Global Polio Eradication Initiative RepositoryEnglish
Supplement 1 to the WHO Polio Laboratory Manual An alternative test algorithm for poliovirus isolation and characterizationWorld Health Organization (WHO)GuidanceEnglish
Supplement 2 to the WHO Polio Laboratory Manual Adaptation of newly received cells to local conditionsWorld Health Organization (WHO)GuidanceEnglish